Figure 3
Effect of xanthurenic acid (XA) on gametocytaemia evaluated by flow cytometry. Experiments were performed on MACS® enriched gametocyte culture. Percentages of each population (M2 and M3) of a gametocyte culture before or after exflagellation with 100 μM xanthurenic acid (XA) were evaluated by flow cytometry (mean data of three independent experiments ± SD, the parasitaemia level varied highly between these three experiments). As no decrease of gametocytaemia was observed in M2 population after XA treatment, this group represented potentially female gametocytes. The decrease observed in the M3 population after XA treatment corresponded to mature male gametocytes exflagellation. The residual M3 population after XA treatment was mostly immature gametocytes and schizonts.