Figure 3

P. yoelii -iRBC do not induce DC maturation in vitro. Bone marrow-derived DC were incubated for 24 h in different conditions. Ratio of red blood cell (RBC):DC is 30:1 (A) CD86 expression of DC co-incubated with iRBC at the schizont stage (Sch), the trophozoite stage (Trophs), a mix of uninfected RBC and rings (Uninf+rings), fixed iRBC, saponized schizonts or isolated food vacuoles (FV). (B) CD86 expression of DC co-incubated with iRBC at the schizont stage (Sch), its lysate, saponized schizonts (Sap sch) or its lysate. An amount of lysate equivalent to whole parasites was added to DC. (C) CD86 expression of DC co-incubated with RBC or iRBC and zymosan. Data correspond to the mean of triplicate samples ± standard deviation.