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Figure 7 | Malaria Journal

Figure 7

From: Dual effect of Plasmodium-infected erythrocytes on dendritic cell maturation

Figure 7

Inhibition of LPS-induced maturation by P. yoelii -infected red blood cells (iRBC) is established between 6 and 18 h. To investigate the kinetics of the iRBC-induced inhibition of DC maturation, DC were pre-incubated for different times (1 to 24 h) with no RBC (white bars), uninfected RBC at 30:1 (grey bars) or iRBC at 30:1 (black bars), and then pulsed with LPS for 18 h. Cells were then analyzed by flow cytometry for expression of CD40 (A) and CD86 (B). To characterize the kinetics of the iRBC-induced inhibition of cytokine production, DC were pre-incubated for different times (0 to 24 h) with uninfected RBC at 30:1 (white bars) or iRBC at 10:1 (grey bars) or 30:1 (black bars), and then pulsed with LPS 1 Ī¼g/ml. Twenty-four hours after addition of LPS, supernatants of DC cultures were collected and IL-12p70 was quantified by ELISA. Data shows a representative experiment out of two independent experiments.

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