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Table 3 Comparison of nested PCR assays using blood, saliva and urine samples with microscopy results as reference.

From: Comparative detection of Plasmodium vivax and Plasmodium falciparum DNA in saliva and urine samples from symptomatic malaria patients in a low endemic area

  Microscopy (%)
Nested PCR P. falciparum P. vivax
  Positive (n = 54) Negative (n = 66) Positive (n = 50) Negative (n = 70)
Blood     
   Positive 54 (100) 15 (22.7) 50 (100) 13 (18.6)
   Negative 0 51 (77.3) 0 57 (81.4)
Saliva (in ethanol)     
   Positive 35 (64.8) 4 (6.1) 40 (80.0) 4 (5.7)
   Negative 19 (35.2) 62 (93.9) 10 (20.0) 66 (94.3)
Saliva (on ice)     
   Positive 24 (44.4) 2 (3.0) 18 (36.0) 2 (2.9)
   Negative 30 (55.6) 64 (97.0) 32 (64.0) 68 (97.1)
Saliva (all)     
   Positive 40 (74.1) 5 (7.6) 42 (84.0) 6 (8.6)
   Negative 14 (25.9) 61 (92.4) 8 (16.0) 64 (91.4)
Urine (in ethanol)     
   Positive 20 (37.0) 0 10 (20.0) 0
   Negative 34 (63.0) 66 (100) 40 (80.0) 70 (100)
Urine (on ice)     
   Positive 9 (16.7) 1 (1.5) 8 (16.0) 0
   Negative 45 (83.3) 65 (98.5) 42 (84.0) 70 (100)
Urine (all)     
   Positive 24 (44.4) 1 (1.5) 17 (34.0) 0
   Negative 30 (55.6) 65 (98.5) 33 (66.0) 70 (100)