Figure 1
Sensitivity of qPCR for detection of pfcrt alleles (CVMNK and CVIET) in P. falciparum DNA controls. The sensitivity of the qPCR was tested in detecting serial dilutions of purified DNA from each control clone. Each control was prepared at a concentration of 3 ng/μl and in ten-fold dilutions to 3 × 10-4 ng/μl. Green fill represents a fluorescent signal above threshold in that channel, grey fill represents no detectable signal above background. CT values are read from the x-axis of the plot, at the point at which the fluorescence curve crosses the threshold. Dd2: CVIET. 3D7: CVMNK. NTC: no template control.