From: Molecular surveillance of Plasmodium vivax dhfr and dhps mutations in isolates from Afghanistan
Gene | Nested-PCR (position) | Primer | Sequence | Temperature °C/time (min) | Product size (bp) | |||||
---|---|---|---|---|---|---|---|---|---|---|
 |  |  |  | A | E | D | C |  | ||
pvdhfr | Nest-1 | VDTOF | ATGGAGGACCTTTCAGATGTATTTGACATT | 64 (2') | 72 (2') | 94 (1') | 25 | 1869 | ||
 |  | VDTOR | GGCGGCCATCTCCATGGTTATTTTATCGTG |  |  |  |  |  | ||
 | Nest-2 (13, 33, 58, 61) | VDF13NF | GACCTTTCAGATGTATTTGACATTTACGGC | 66 (2') | 72 (2') | 94 (1') | 25 | 232 | ||
 |  | VDF13NR | GGTACCTCTCCCTCTTCCACTTTAGCTTCT |  |  |  |  |  | ||
 | Nest-2 (57, 117) | VDNF57 | CATGGAAATGCAACTCCGTCGATATGATGT | 66 (2') | 72 (2') | 94 (1') | 25 | 472 | ||
 |  | VDFNR | TCACACGGGTAGGCGCCGTTGATCCTCGTG |  |  |  |  |  | ||
 | Nest-2 (57, 173) | VDTOF | ATGGAGGACCTTTCAGATGTATTTGACATT | 66 (2') | 72 (2') | 94 (1') | 25 | 608 | ||
 |  | VDFNR | TCACACGGGTAGGCGCCGTTGATCCTCGTG |  |  |  |  |  | ||
pvdhps | Nest-1 | VDHPSOF | ATTCCAGAGTATAAGCACAGCACATTTGAG | 58 (2') | 72 (1') | 94 (1') | 21 | 1499 | ||
 |  | VDHPSOR | CTAAGGTTGATGTATCCTTGTGAGCACATC |  |  |  |  |  | ||
 | Nest-2 (383) | VDHPSNF | AATGGCAAGTGATGGGGCGAGCGTGATTGA | 50 (2') | 72 (2') | 94 (1') | 25 | 703 | ||
 |  | VDHPSNR | CAGTCTGCACTCCCCGATGGCCGCGCCACC |  |  |  |  |  | ||
 | Nest-2 (553) | VDHPS553OF | TTCTCTTTGATGTCGGCCTGGGGTTGGCCA | 68 (1') | 72 (1') | 94 (1') | 30 | 170 | ||
 |  | VDHPSNR | CAGTCTGCACTCCCCGATGGCCGCGCCACC |  |  |  |  |  |