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Table 1 Primers and profiles used for amplification of the pvdhfr and pvdhps genes.

From: Molecular surveillance of Plasmodium vivax dhfr and dhps mutations in isolates from Afghanistan

Gene

Nested-PCR

(position)

Primer

Sequence

Temperature °C/time (min)

Product size (bp)

    

A

E

D

C

 

pvdhfr

Nest-1

VDTOF

ATGGAGGACCTTTCAGATGTATTTGACATT

64 (2')

72 (2')

94 (1')

25

1869

  

VDTOR

GGCGGCCATCTCCATGGTTATTTTATCGTG

     
 

Nest-2

(13, 33, 58, 61)

VDF13NF

GACCTTTCAGATGTATTTGACATTTACGGC

66 (2')

72 (2')

94 (1')

25

232

  

VDF13NR

GGTACCTCTCCCTCTTCCACTTTAGCTTCT

     
 

Nest-2

(57, 117)

VDNF57

CATGGAAATGCAACTCCGTCGATATGATGT

66 (2')

72 (2')

94 (1')

25

472

  

VDFNR

TCACACGGGTAGGCGCCGTTGATCCTCGTG

     
 

Nest-2

(57, 173)

VDTOF

ATGGAGGACCTTTCAGATGTATTTGACATT

66 (2')

72 (2')

94 (1')

25

608

  

VDFNR

TCACACGGGTAGGCGCCGTTGATCCTCGTG

     

pvdhps

Nest-1

VDHPSOF

ATTCCAGAGTATAAGCACAGCACATTTGAG

58 (2')

72 (1')

94 (1')

21

1499

  

VDHPSOR

CTAAGGTTGATGTATCCTTGTGAGCACATC

     
 

Nest-2

(383)

VDHPSNF

AATGGCAAGTGATGGGGCGAGCGTGATTGA

50 (2')

72 (2')

94 (1')

25

703

  

VDHPSNR

CAGTCTGCACTCCCCGATGGCCGCGCCACC

     
 

Nest-2

(553)

VDHPS553OF

TTCTCTTTGATGTCGGCCTGGGGTTGGCCA

68 (1')

72 (1')

94 (1')

30

170

  

VDHPSNR

CAGTCTGCACTCCCCGATGGCCGCGCCACC

     
  1. A: Annealing, E: Extension, D: Denaturation, C: Cycles