From: Molecular surveillance of Plasmodium vivax dhfr and dhps mutations in isolates from Afghanistan
Gene | Primers | RFLP Position | Restriction Enzyme | Company | Uncut Product Size (bp) | Cut Product Size (bp) |
---|---|---|---|---|---|---|
pvdhfr | VDF13NF/VDF13NR | I13L | Hae III | Roche | 232 | L: 200 + 32 |
 |  | P33L | Cfr42I (Sac II) | Fermentas | 232 | P: 138 + 94 |
 |  | S58R | Alu I | Fermentas | 232 | S: 167 + 40 + 25 R: 207 + 25 |
 |  | T61M | Tsp45 I | BioLabs | 232 | T: 200 + 32 |
 | VDTOF/VDFNR | F57I/L | Xmn I | Fermentas | 608 | F: 166 + 442 |
 |  | I173L | Eco130 I (Sty I) | Fermentas | 608 | L: 438 + 97 + 73 I: 472 + 136 |
 | VDNF57/VDFNR | F57I/L | BsrG I | BioLabs | 472 | I: 444 + 28 |
 |  | S117N/T | Pvu II | BioLabs | 472 | S: 258 + 214 |
 |  | S117N/T | Bsr I | Fermentas | 472 | N: 219 + 253 |
 |  | S117N/T | BstN I | BioLabs | 472 | T: 257 + 215 |
Pvdhps | VDHPSNF/VDHPSNR | A383G | Msp I (Hpa II) | Fermentas | 703 | G: 655 + 48 |
 | VDHPS553OF/VDHPSNR | A553G | MscI | BioLabs | 170 | A:143 + 27 |