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Table 2 RFLP protocols used for genotyping pvdhfr and pvdhps genes.

From: Molecular surveillance of Plasmodium vivax dhfr and dhps mutations in isolates from Afghanistan

Gene

Primers

RFLP Position

Restriction Enzyme

Company

Uncut Product Size (bp)

Cut Product

Size (bp)

pvdhfr

VDF13NF/VDF13NR

I13L

Hae III

Roche

232

L: 200 + 32

  

P33L

Cfr42I (Sac II)

Fermentas

232

P: 138 + 94

  

S58R

Alu I

Fermentas

232

S: 167 + 40 + 25

R: 207 + 25

  

T61M

Tsp45 I

BioLabs

232

T: 200 + 32

 

VDTOF/VDFNR

F57I/L

Xmn I

Fermentas

608

F: 166 + 442

  

I173L

Eco130 I (Sty I)

Fermentas

608

L: 438 + 97 + 73

I: 472 + 136

 

VDNF57/VDFNR

F57I/L

BsrG I

BioLabs

472

I: 444 + 28

  

S117N/T

Pvu II

BioLabs

472

S: 258 + 214

  

S117N/T

Bsr I

Fermentas

472

N: 219 + 253

  

S117N/T

BstN I

BioLabs

472

T: 257 + 215

Pvdhps

VDHPSNF/VDHPSNR

A383G

Msp I (Hpa II)

Fermentas

703

G: 655 + 48

 

VDHPS553OF/VDHPSNR

A553G

MscI

BioLabs

170

A:143 + 27