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Figure 5 | Malaria Journal

Figure 5

From: Antibodies against multiple merozoite surface antigens of the human malaria parasite Plasmodium falciparum inhibit parasite maturation and red blood cell invasion

Figure 5

Specificity of processing inhibition by antibodies targeting MSP-1/MSP-6/MSP-7 or AMA-1. Processing of AMA-1 and of MSP-1 was detected by western blot using mAb 4G2 and mAb X509 respectively (to identify the processing products AMA-144/48 and MSP-133 respectively). 'Start' indicates samples taken at the beginning of the assay, before incubation of merozoites. (A) Incubation of isolated merozoites in the absence of serum (control), or in the presence of normal mouse sera, (NMS1 and NMS2), or with polyclonal mouse sera raised against reduced and alkylated (R2-R5) or correctly folded (N2-N5) PfAMA-1 ectodomain. Additionally, purified mAb 4G2 was tested for processing inhibition. (B) Dependency of processing on antibody concentration. Merozoites were either incubated in the absence of the sera (tracks 2, 3 and 4 from the left) or in the presence of varying amounts of N3 or N5 serum - presented in terms of percentage (v/v) of serum. The two sets of panels show western blot results of two different loadings of supernatant samples on SDS-PAGE. (C) Processing in presence of AMA-1 antiserum N5, as well as monoclonal antibodies mAb 12.8, mAb 12.10, mAb 7.5 specific for MSP-119, mAb 89.1 specific for MSP-183 and control mAb 4F3. (D) Inhibition of AMA-1 processing by varying amounts of αAMA-1 serum N5, and inhibition of MSP-142 processing by varying amounts of mAb 12.8. Inhibition of processing of both AMA-1 and MSP-1 in the presence of antibodies against both proteins (track 11).

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