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Figure 1 | Malaria Journal

Figure 1

From: Up- and down-modulation of liver cytochrome P450 activities and associated events in two murine malaria models

Figure 1

Effects of P.berghei infection on CYP activities, NO production and liver weights in DBA-2 mice. Time course of changes of liver CYP activities (CYP1a: EROD, 2b: BROD, 2a5: COH), NO serum levels, and liver and spleen weights in DBA-2 mice infected with P.berghei (ANKA). Panel A shows NO serum levels (μM) and parasitaemia rates (%PE). Results in Panel B (enzyme activities) and C (liver and spleen weights) are shown as % of mean values for non-infected controls (day 0 = 100) and comparisons were made by ANOVA and Dunnett's post-hoc test. In panels B and C statistical evaluation was made with untransformed data. Differences (P < 0.05) from non-infected control group (day 0) are indicated by an asterisk (*) superscript. Panels B and C control values (100%) are as follows (mean ± S.E.M.): EROD = 94.7 ± 41 pmol resorufin/mg protein/min; BROD = 74.6 ± 21 pmol resorufin/mg protein/min; COH = 193.9 ± 27 pmol umbelliferone/mg protein/min; liver wt = 0.99 ± 0.02 g; spleen wt = 0.08 ± 0.04 g. Control NO = 35.4 ± 2.8 μM (Panel A). Numbers of mice used (N) are as follows: PID0 = 15; PID4 = 10; PID8 = 10; PID12 = 10; PID16 = 11; PID20 = 14. Of 9 infected mice that were not killed (not included), 2 died on PID22, and 7 on PID24. No infected DBA-2 exhibited neurological signs of cerebral malaria.

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