Effects of P. c. chabaudi infection on CYP activities, NO production and liver weights in C57BL/6 mice. Time course of changes of liver CYP activities (CYP1a: EROD, 2b: BROD, 2a5: COH), NO serum levels, and liver and spleen weights in C57BL/6 mice infected with P. chabaudi chabaudi. Panel A shows NO serum levels (μM) and parasitaemia rates (%PE). Results in Panel B (enzyme activities) and C (liver and spleen weights) are shown as % of mean values for non-infected controls (day 0 = 100) and comparisons were made by ANOVA and Dunnett's post-hoc test. In panels B and C statistical evaluation was made with untransformed data. Differences (P < 0.05) from non-infected control group (day 0) are indicated by an asterisk (*) superscript. Panels B and C control values (100%) are as follows (mean ± S.E.M.): EROD = 182.4 ± 5.8 pmol resorufin/mg protein/min; BROD = 121.2 ± 6.0 pmol resorufin/mg protein/min; COH = 16.5 ± 1.2 pmol umbelliferone/mg protein/min; liver wt = 0.84 ± 0.02 g; spleen wt = 0.07 ± 0.003 g. Control NO = 51.12 ± 2.36 μM (Panel A). Numbers of mice used (N) are as follows: PID0 = 10; PID2 = 14; PID5 = 10; PID6 = 6, PID10 = 6; PID14 = 6; PID19 = 8.