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Figure 3 | Malaria Journal

Figure 3

From: Structure, localization and histone binding properties of nuclear-associated nucleosome assembly protein from Plasmodium falciparum

Figure 3

Stage specific expression of PfNapS-GFP chimeras (a). Deletion constructs of PfNapS-GFP. (b) Western blot analysis of transgenic P. falciparum cell lines expressing GFP-tagged versions of PfNapS with antibodies against GFP. PfHsp-70 expression was detected as a loading control. Localization of (c) PfNapS-full length-GFP (d) aa1-30-GFP (e) aa30-90-GFP and (f) aa90-269-GFP in parasite merozoite, ring, trophozoite, late-trophozoite and schizont stages. The first column shows DIC (Differential Interference Contrast) images, followed by images with a nuclear DAPI stain, GFP fluorescence, an overlay of the nuclear and the GFP localization and overlay of all four images.

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