Figure 2
From: Apoptosis stalks Plasmodium falciparum maintained in continuous culture condition
Panel A and B: Level of fragmented DNA using terminal deoxynucleotidyl-transferase-mediated dUTP nick-end labeling (TUNEL). Evidence of DNA fragmentation as measured by TUNEL is discernible albeit at very low level by fluorescent microscopy (Panel A) and flow cytometry (Panel B) at seeding parasitaemia, but increases dramatically by the time the parasite culture crashes. Panel C: Dot blots showing percentage accumulation of TMRE in the mitochondria of trophozoites at different parasitaemia. As shown, the proportion of cells with positive TMRE staining was maximal at 3.7% parasitaemia and declined thereafter.