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Table 1 Abdominal status and human blood indices (HBI) determined by molecular assays of visually fed and unfed anophelines at three field sites in southern Africa

From: Underestimation of foraging behaviour by standard field methods in malaria vector mosquitoes in southern Africa

Site An. vector species Fed visually (%) Visually Fed and confirmed molecularly # (%) HBI * Visually Unfed but Fed by Kent PCR (%) Visually Unfed, unfed by Kent PCR, Fed by Fornadel PCR (%) Overall PCR confirmed Fed a (%) Under-estimation of blood feeding frequency b (%) Updated HBI c
Macha (n = 643) arabiensis (n = 643) 11.7 7.5 0.96 3.9 11.5 22.1 10.4 0.87
Nchelenge Mar-Apr 2012 (n = 411) funestus s.s. (n = 343) 32.4 32.4 1.00 0.86 8.26 38.5 6.1 1.00
gambiae s.s. (n = 36) 25.0 25.0 1.00 3.7 15.4 38.9 13.9 1.00
leesoni (n = 32) 18.8 18.8 0.75 7.7 16.7 37.5 18.7 0.80
Mutasa Dec 2012 (n = 84) funestus s.s. (n = 84) 30.5 30.5 0.96 0.0 5.26 35.4 4.9 0.93
leesoni (n = 2) 0.0 0.0 --- 0.0 0.0 0.0 --- ---
  1. Restricted to specimens detected to be anthropophilic.
  2. #Confirmation by both Kent and Fornadel PCRs.
  3. *HBI based on visually and molecularly confirmed fed mosquitoes.
  4. aCombined results of Kent and Fornadel PCRs run on visually fed and unfed mosquitoes.
  5. bDifference in PCR confirmed blood feeding frequency between visually fed mosquitoes only and both visually unfed and fed mosquitoes.
  6. cHBI based on molecularly determined fed mosquitoes.