Table 1 Primer sequences and PCR reaction conditions to amplify pfhrp2 , pfhrp3 and their respective flanking genes
From: Prevalence of pfhrp2 and pfhrp3 gene deletions in Puerto Lempira, Honduras
Gene | Reaction | Primer name | Primer sequence | Annealing temperature, X (°C) | Expected amplicon size (bp) |
|---|---|---|---|---|---|
PF3D7_0831900 (MAL7P1.230) | Primary | 230 F1 | 5′ GAT ATC ATT AGA AAA CAA GAG CTT AG 3′ | 63 | 301 |
230R | 5′ TAT CCA ATC CTT CCT TTG CAA CAC C 3′ | ||||
Nested | 230 F | 5′ TAT GAA CGC AAT TTA AGT GAG GCA G 3′ | 65 | ||
230R | 5′ TAT CCA ATC CTT CCT TTG CAA CAC C 3′ | ||||
PfHRP2 Exon 1–2, PF3D7_0831800 | Primary | 2E12F1 | 5′ GGT TTC CTT CTC AAA AAA TAA AG 3′ | 55 | 228 |
2E12R1 | 5′ TCT ACA TGT GCT TGA GTT TCG 3′ | ||||
Nested | 2E12F | 5′ GTA TTA TCC GCT GCC GTT TTT GCC 3′ | 62 | ||
2E12R | 5′ CTA CAC AAG TTA TTA TTA AAT GCG GAA 3′ | ||||
PF3D7_0831700 (MAL7P1.228) | Primary | 228 F | 5′ AGA CAA GCT ACC AAA GAT GCA GGT G 3′ | 60 | 198 |
228R | 5′ TAA ATG TGT ATC TCC TGA GGT AGC 3′ | ||||
Nested | 228 F1 | 5′ CCA TTG CTG GTT TAA ATG TTT TAA G 3′ | 63 | ||
228R | 5′ TAA ATG TGT ATC TCC TGA GGT AGC 3′ | ||||
PF3D7_1372100, (MAL13P1.485) | Primary | 485 F | 5′ TTG AGT GCA ATG ATG AGT GGA G 3′ | 60 | 241 |
485R | 5′ AAA TCA TTT CCT TTT ACA CTA GTG C 3′ | ||||
Nested | 485 F1 | 5′ GTT ACT ACA TTA GTG ATG CAT TC 3′ | 59 | ||
485R | 5′ AAA TCA TTT CCT TTT ACA CTA GTG C 3′ | ||||
PfHRP3 Exon 1–2, PF3D7_1372200 | Primary | 3E12F1 | 5′ GGT TTC CTT CTC AAA AAA TAA AA 3′ | 53 | 225 |
3E12R1 | 5′ CCT GCA TGT GCT TGA CTT TA 3′ | ||||
Nested | 3E12F | 5′ ATA TTA TCG CTG CCG TTT TTG CT 3′ | 62 | ||
3E12R | 5′ CTA AAC AAG TTA TTG TTA AAT TCG GAG 3′ | ||||
PF3D7_1372400 (MAL13P1.475) | Primary | 475 F | 5′ TTC ATG AGT AGA TGT CCT AGG AG 3′ | 55 | 212 |
475R | 5′ TCG TAC AAT TCA TCA TAC TCA CC 3′ | ||||
Nested | 475 F | 5′ TTC ATG AGT AGA TGT CCT AGG AG 3′ | 61 | ||
475R1 | 5′ GGA TGT TTC GAC ATT TTC GTC G 3′ |