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Table 1 Primer sequences and PCR reaction conditions to amplify pfhrp2 , pfhrp3 and their respective flanking genes

From: Prevalence of pfhrp2 and pfhrp3 gene deletions in Puerto Lempira, Honduras

Gene Reaction Primer name Primer sequence Annealing temperature, X (°C) Expected amplicon size (bp)
PF3D7_0831900 (MAL7P1.230) Primary 230 F1 5′ GAT ATC ATT AGA AAA CAA GAG CTT AG 3′ 63 301
230R 5′ TAT CCA ATC CTT CCT TTG CAA CAC C 3′
Nested 230 F 5′ TAT GAA CGC AAT TTA AGT GAG GCA G 3′ 65
230R 5′ TAT CCA ATC CTT CCT TTG CAA CAC C 3′
PfHRP2 Exon 1–2, PF3D7_0831800 Primary 2E12F1 5′ GGT TTC CTT CTC AAA AAA TAA AG 3′ 55 228
2E12R1 5′ TCT ACA TGT GCT TGA GTT TCG 3′
Nested 2E12F 5′ GTA TTA TCC GCT GCC GTT TTT GCC 3′ 62
2E12R 5′ CTA CAC AAG TTA TTA TTA AAT GCG GAA 3′
PF3D7_0831700 (MAL7P1.228) Primary 228 F 5′ AGA CAA GCT ACC AAA GAT GCA GGT G 3′ 60 198
228R 5′ TAA ATG TGT ATC TCC TGA GGT AGC 3′
Nested 228 F1 5′ CCA TTG CTG GTT TAA ATG TTT TAA G 3′ 63
228R 5′ TAA ATG TGT ATC TCC TGA GGT AGC 3′
PF3D7_1372100, (MAL13P1.485) Primary 485 F 5′ TTG AGT GCA ATG ATG AGT GGA G 3′ 60 241
485R 5′ AAA TCA TTT CCT TTT ACA CTA GTG C 3′
Nested 485 F1 5′ GTT ACT ACA TTA GTG ATG CAT TC 3′ 59
485R 5′ AAA TCA TTT CCT TTT ACA CTA GTG C 3′
PfHRP3 Exon 1–2, PF3D7_1372200 Primary 3E12F1 5′ GGT TTC CTT CTC AAA AAA TAA AA 3′ 53 225
3E12R1 5′ CCT GCA TGT GCT TGA CTT TA 3′
Nested 3E12F 5′ ATA TTA TCG CTG CCG TTT TTG CT 3′ 62
3E12R 5′ CTA AAC AAG TTA TTG TTA AAT TCG GAG 3′
PF3D7_1372400 (MAL13P1.475) Primary 475 F 5′ TTC ATG AGT AGA TGT CCT AGG AG 3′ 55 212
475R 5′ TCG TAC AAT TCA TCA TAC TCA CC 3′
Nested 475 F 5′ TTC ATG AGT AGA TGT CCT AGG AG 3′ 61
475R1 5′ GGA TGT TTC GAC ATT TTC GTC G 3′
  1. All reactions were conducted using the following reaction conditions: 95°C/5 min – [95°C/30 sec; X/30 sec; 68°C/30 sec] × 30 cycles – 68°C/5 min – 4°C/∞. The old PlasmoDB gene IDs are included in parentheses below the current IDs.