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Table 1 Primers and probes used for screening and identification of Plasmodium species

From: Molecular detection of human Plasmodium species in Sabah using PlasmoNex™ multiplex PCR and hydrolysis probes real-time PCR

Species

Primer or probe

Conc. [nM]

Sequence (5’ → 3’) b

References

Plasmodium spp.

Plasmo1

200

GTTAAGGGAGTGAAGACGATCAGA

[10]

Plasmodium spp.

Plasmo2

200

AACCCAAAGACTTTGATTTCTCATAA

Plasmodium spp.

Plasprobe

50

FAM-ACCGTCGTAATCTTAACCATAAA

CTATGCCGACTAG-BHQ-1

P. falciparum

Fal-F primer

200

CCGACTAGGTGTTGGATGAAAGTGTTAA

[10,12]

P. falciparum

Falcprobea

80

Cy5-AGCAATCTAAAAGTCACCTCGAA

AGATGACT-BHQ-1

P. vivax

Viv-F primer

50

CCGACTAGGCTTTGGATGAAAGATTTTA

P. vivax

Vivprobea

80

TR-AGCAATCTAAGAATAAACTCCGA

AGAGAAAATTCT-BHQ-2

P. malariae

Mal-F primer

50

CCGACTAGGTGTTGGATGATAGAGTAAA

P. malariae

Malaprobe

50

FAM-CTATCTAAAAGAAACACTCAT-MGBNFQ

P. knowlesi

Pkprobe

80

FAM-CTCTCCGGAGATTAGAACTCTTA

[14]

GATTGCT-BHQ-1

Human

β2M-F

900

TGAGTATGCCTGCCGTGTGA

[15]

Human

β2M-R

900

ACTCATACACAACTTTCAGCAGCTTAC

Human

β2M-probe

100

FAM-CCATGTGACTTTGTCACAGCCCA

AGATAGTT-TAMRA

  1. aProbe sequence is as previously published [11], with modified fluorophores.
  2. bTAMRA, 6-carboxytetramethylrhodamine; MGBNFQ, minor groove binding nonfluorescent quencher; BHQ, black hole quencher; Cy5, cyanine; FAM, carboxyfluorescein; TR, Texas Red.