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Table 3 The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and disease prevalence (DP) of the real-time PCR compared to PlasmoNex TM PCR

From: Molecular detection of human Plasmodium species in Sabah using PlasmoNex™ multiplex PCR and hydrolysis probes real-time PCR

PlasmoNex™ as standard (Confirmed by sequencing)
Species P. vivax P. falciparum P. knowlesi
Test* Positive Negative Positive Positive Negative Positive
Species-specific real-time PCR Positive 16 0 35 2 149 0
Negative 6 185 0 170 5 53
  Percentage 95% CI Percentage 95% CI Percentage 95% CI
Sensitivity 72.73 49.78 to 89.20 100.00 89.90 to 100.00 96.75 92.58 to 98.93
Specificity 100.00 98.01 to 100.00 98.84 95.85 to 99.83 100.00 93.21 to 100.00
PPV 100.00 79.24 to 100.00 94.59 81.77 to 99.18 100.00 97.53 to 100.00
NPV 96.86 93.28 to 98.83 100.00 97.83 to 100.00 91.38 81.01 to 97.11
DP 10.63 6.78 to 15.65 16.91 12.07 to 22.72 74.40 67.88 to 80.19
  1. *Included two triple-species mixed infections and five none speciation samples.