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Table 3 The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and disease prevalence (DP) of the real-time PCR compared to PlasmoNex TM PCR

From: Molecular detection of human Plasmodium species in Sabah using PlasmoNex™ multiplex PCR and hydrolysis probes real-time PCR

PlasmoNex™ as standard (Confirmed by sequencing)

Species

P. vivax

P. falciparum

P. knowlesi

Test*

Positive

Negative

Positive

Positive

Negative

Positive

Species-specific real-time PCR

Positive

16

0

35

2

149

0

Negative

6

185

0

170

5

53

 

Percentage

95% CI

Percentage

95% CI

Percentage

95% CI

Sensitivity

72.73

49.78 to 89.20

100.00

89.90 to 100.00

96.75

92.58 to 98.93

Specificity

100.00

98.01 to 100.00

98.84

95.85 to 99.83

100.00

93.21 to 100.00

PPV

100.00

79.24 to 100.00

94.59

81.77 to 99.18

100.00

97.53 to 100.00

NPV

96.86

93.28 to 98.83

100.00

97.83 to 100.00

91.38

81.01 to 97.11

DP

10.63

6.78 to 15.65

16.91

12.07 to 22.72

74.40

67.88 to 80.19

  1. *Included two triple-species mixed infections and five none speciation samples.