Comparison of a PfHRP2-based rapid diagnostic test and PCR for malaria in a low prevalence setting in rural southern Zambia: implications for elimination

Table 1 Comparison of Pf HRP2-based RDT results with microscopy and nested PCR from 2009 – 2012

	Microscopy positive	Microscopy negative	Total	PCR positive	PCR negative	Total
RDT positive	2	8	10	8	2	10
RDT negative	2	3280	3282	39	2888	2927
Total	4	3288	3292	47	2890	2937*

*Of the 2,975 enrolled during this period, 2,937 (99%) were tested by both RDT and nested PCR.
Sensitivity of PfHRP2-based RDT compared to microscopy = 0.50.
Specificity of PfHRP2-based RDT compared to microscopy = > 0.99.
Positive predictive value of PfHRP2-based RDT compared to microscopy = 20%.
Negative predictive value of PfHRP2-based RDT compared to microscopy = > 99%.
Sensitivity of PfHRP2-based RDT compared to PCR = 0.17.
Specificity of PfHRP2-based RDT compared to PCR = > 0.99.
Positive predictive value of PfHRP2-based RDT compared to PCR = 80%.
Negative predictive value of PfHRP2-based RDT compared to PCR = 99%.

ISSN: 1475-2875