Figure 6

PCR/RPLF genotypes and allelic frequencies of 27 P. vivax Hainan isolates based on Pvmsp-3α and Pvmsp-3β. (A) The amplification products of Pvmsp-3α observed in Hainan P. vivax isolates were digested by Alu I, and nine variants were obtained (A1 to A8, B1). (B) The amplification products of Pvmsp-3α observed in Hainan P. vivax isolates were digested by Hha I, and eleven variants were obtained (H1 to H11). The lane with the molecular weight marker (50 bp ladder) is labeled as M1 and that of named D2000 marker do as M2. (C) The amplification products of Pvmsp-3β observed in Hainan P. vivax isolates were digested by Pst I, and ten variants were obtained (PA1 to PA6, PB1 to PB3, and U). The lane with the molecular weight marker (50 bp ladder) is labeled as M1 and that of named D2000 marker done as M2.