|
pvcsp(N1)
|
F:5′-ATGTAGATCTGTCCAAGGCCATAAA-3′
|
95°C 3 min/[94°C 30 s, 58°C 30 s, 72°C 1.5 min] × 30 cycles, 72°C 5 min
|
|
R:5′-TAATTGAATAATGCTAGGACTAACAATATG-3′
|
|
pvcsp(N2)
|
F:5′-CCAGATGACGAGGAAGGAGATGC-3′
|
95°C 3 min/[94°C 30 s, 58°C 30 s, 72°C 1 min] × 35 cycles, 72°C 5 min
|
|
R:5′-TCTTTCACAGACTTTTCATTTGGG-3′
|
|
pvmsp-1(N1)
|
F:5′-GAGCCCTACTACTTGATGGTCC-3′
|
95°C 3 min/[94°C 30 s, 58°C 30 s, 72°C1min] × 35 cycles, 72°C 5 min
|
|
R:5′-CCTTCTGGTACAGCTCAATG-3′
|
|
pvmsp-3α(N1)
|
F:5′-CAGCAGACACCATTTAAGG-3′
|
95°C 3 min/[94°C 30 s, 54°C 30 s, 68°C 2.5 min] × 30 cycles, 68°C 5 min
|
|
R:5′-CCGTTTGTTGATTAGTTGC-3′
|
|
pvmsp-3α(N2)
|
F:5′-GACCAGTGTGATACCATTAACC-3′
|
95°C 3 min/[94°C 30 s, 55°C 30 s, 68°C 2.5 min] × 40 cycles, 68°C 5 min
|
|
R:5′-ATACTGGTTCTTCGTCTTCAGG-3′
|
|
pvmsp-3β(N1)
|
F:GTATTCTTCGCAACACTC
|
95°C 3 min/[94°C 30 s, 54°C 30 s, 68°C 2.5 min] × 30 cycles, 68°C 5 min
|
|
R:CTTCTGATGTTATTTCCAG
|
|
pvmsp-3β(N2)
|
F:CGAGGGGCGAAATTGTAAACC
|
95°C 3 min/[94°C 30 s, 55°C 30 s, 68°C 2.5 min] × 40 cycles, 68°C 5 min
|
|
R:GCTGCTTCTTTTGCAAAGG
|
