Skip to main content

Table 1 Primers for amplication on pvmsp - , pvmsp -3 β , pvmsp -1, and pvcsp genes

From: Genetic diversity of Plasmodium vivax population before elimination of malaria in Hainan Province, China

Gene Primers*

Squencen(5′-3′)

PCR cycling conditions**

pvcsp(N1)

F:5′-ATGTAGATCTGTCCAAGGCCATAAA-3′

95°C 3 min/[94°C 30 s, 58°C 30 s, 72°C 1.5 min] × 30 cycles, 72°C 5 min

R:5′-TAATTGAATAATGCTAGGACTAACAATATG-3′

pvcsp(N2)

F:5′-CCAGATGACGAGGAAGGAGATGC-3′

95°C 3 min/[94°C 30 s, 58°C 30 s, 72°C 1 min] × 35 cycles, 72°C 5 min

R:5′-TCTTTCACAGACTTTTCATTTGGG-3′

pvmsp-1(N1)

F:5′-GAGCCCTACTACTTGATGGTCC-3′

95°C 3 min/[94°C 30 s, 58°C 30 s, 72°C1min] × 35 cycles, 72°C 5 min

R:5′-CCTTCTGGTACAGCTCAATG-3′

pvmsp-(N1)

F:5′-CAGCAGACACCATTTAAGG-3′

95°C 3 min/[94°C 30 s, 54°C 30 s, 68°C 2.5 min] × 30 cycles, 68°C 5 min

R:5′-CCGTTTGTTGATTAGTTGC-3′

pvmsp-(N2)

F:5′-GACCAGTGTGATACCATTAACC-3′

95°C 3 min/[94°C 30 s, 55°C 30 s, 68°C 2.5 min] × 40 cycles, 68°C 5 min

R:5′-ATACTGGTTCTTCGTCTTCAGG-3′

pvmsp-3β(N1)

F:GTATTCTTCGCAACACTC

95°C 3 min/[94°C 30 s, 54°C 30 s, 68°C 2.5 min] × 30 cycles, 68°C 5 min

R:CTTCTGATGTTATTTCCAG

pvmsp-3β(N2)

F:CGAGGGGCGAAATTGTAAACC

95°C 3 min/[94°C 30 s, 55°C 30 s, 68°C 2.5 min] × 40 cycles, 68°C 5 min

R:GCTGCTTCTTTTGCAAAGG

  1. N1 = Nest 1 (Primary) reaction; N2 = Nest 2 (Secondary) PCR reaction.
  2. *F = Forward primer; R = Reverse primer. The reference sources of the primers are indicated.
  3. **The cycling conditions have been modified in the present work.
  4. The two columns indicate conditions for the primary and secondary amplification reaction.