Table 2 PCR primers used and expected product size in malaria diagnosis
From: Establishing a China malaria diagnosis reference laboratory network for malaria elimination
Reaction | Primer name | Primer sequence | Product length |
|---|---|---|---|
First PCR | rPLU5 | 5′-CCTGTTGTTGCCTTAAACTTC-3′ | 1200 bp |
rPLU6 | 5′-TTAAAATTGTTGCAGTTAAAACG-3′ | ||
Nested PCR | rFAL1 | 5′-TTAAACTGGTTTGGGAAAACCAAATATATT-3′ | 205 bp |
rFAL2 | 5′-ACACAATGAACTCAATCATGACTACCCGTC-3′ | ||
rVIV1 | 5′-CGCTTCTAGCTTAATCCACATAACTGATAC-3′ | 120 bp | |
rVIV2 | 5′-ACTTCCAAGCCGAAGCAAAGAAAGTCCTTA-3′ | ||
rMAL1 | 5′-ATAACATAGTTGTACGTTAAGAATAACCGC-3′ | 141 bp | |
rMAL2 | 5′-AAAATTCCCATGCATAAAAAATTATACAAA-3′ | ||
rOVA1 | 5′-ATCTCTTTTGCTATTTTTTAGTATTGGAGA-3′ | 800 bp | |
rOVA2 | 5′-GGAAAAGGACACATTAATTGTATCCTAGTG-3′ |