Skip to main content

Advertisement

Table 3 Diagnostic accuracy of NINA-LAMP and microscopy compared with the gold standard nested PCR for diagnosis of Plasmodium parasite and P. falciparum at Kola Diba Health Centre, Northwest Ethiopia, 2014

From: Evaluation of non-instrumented nucleic acid amplification by loop-mediated isothermal amplification (NINA-LAMP) for the diagnosis of malaria in Northwest Ethiopia

Method   Senstivity(%) (95% CI) Specificity(%) (95% CI) PPV(%) (95% CI) NPV(%) (95% CI) %agreement (Kapp = κ )
NINA -LAMP Pan (Gondar) 96.8 (83.2-99.5) 84.3 (71.4-92.9) 52.1 (33.9-71.3) 99.3 (96.0-99.9) 89.0 (0.776)
  Pf (Gondar) 100 (75.1-100) 81.2 (69.9-89.6) 27.0 (14.8-40.0) 100 (97.6-100) 84.1 (0.577)
  Pan (Calgary) 96.8 (83.2-99.5) 98.0 (89.5-99.7) 89.7 (58.3-98.2) 99.4 (96.8-99.9) 97.6 (0.948)
  Pf (Calgary) 100 (75.1-100) 100 (94.7-100) 100 (49.8-100) 100 (98.2-100) 100 (1.000)
Microscopy Pan 93.6 (78.5-99.0) 98.0 (89.5- 99.7) 89.4 (56.9-98.2) 98.8 (95.9-99.8) 96.3 (0.922)
  Pf 92.3 (63.9-98.7) 100 (94.7-100 ) 100 (45.8-100) 99.5 (97.4-99.9) 98.8 (0.953)
  1. Percentage of agreement between tests: (Number of positives by both tests + Number of negatives by both tests)/N, against what might be expected by chance; Kappa value(κ): 1.00 = Perfect agreement; > 0.80, almost perfect agreement; 0.61-0.8, substantial agreement; 0.41-0.6, moderate agreement; 0.21-0.4, fair agreement; NPV = negative predictive value; PPV = positive predictive value; Pan = Plasmodium genus; Pf = Plasmodium falciparum; 95% CI = 95% confidence interval.