Figure 3

Validation of SYBR Green results by Microscopy and estimation of quenching of fluorescence by plant extracts. (a) Micrographs of synchronized ring stage parasite cultures treated with ethyl acetate extract of Anisomeles malabarica leaf and Psidium guajava leaf after 48 h. Note that at 12.5 μg/ml, the parasitemia is decreased and the parasite is arrested at trophozoite stage in case of Pg while the arrest in case of Am is at early schizont stage. (b) Examination of fluorescence quenching effects in plant extracts: Untreated and extract treated (100 μg/ml) parasite cultures (10% parasitemia) were subjected to SYBR Green fluorescence intensity measurements. The nearly identical intensities across untreated control and extract treated test samples indicate that the test extracts showed no significant quenching of SYBR green 1-DNA fluorescence.