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Figure 2 | Malaria Journal

Figure 2

From: Fast track antibody V-gene rescue, recombinant expression in plants and characterization of a PfMSP4-specific antibody

Figure 2

Sequence alignment of the rescued antibody sequence and the corresponding germ-line sequence. Antibody V regions were amplified from cDNA isolated from the hybridoma cell line 2.44 using specific primers for the VH and VL regions. The protein-sequence alignment included the most closely related germ-line V gene indicated by the IMGT/V-quest tool. For both, the VH (A) and VL (B) domains, CDRs 1–3 were determined according to Kabat definitions [51] and are highlighted in boxes.

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