Molecular diagnosis of
infection by nested-PCR of 30 non-human primate samples. Molecular diagnosis of Plasmodium infection by nested-PCR in 30 non-human primates from CPRJ using specific primers for P. falciparum (Pf), P. vivax (Pv) and Plasmodium malariae (Pm). Plasmodium simium was identified in Sapajus xanthosternos (2324), Sapajus robustus (2209) and Cebus sp. (2503 and 2559); and P. brasilianum was detected in Sapajus xanthosternos (2005 and 2539), Callicebus personatus (2466), Aotus nigriceps (2203), Sapajus robustus (2209) and Alouatta g. clamitans (2443). Agarose gels, 1.5% stained with ethidium bromide. Numbers above gels indicated the non-human primates according to Additional file 1. Pf+, Pv + and Pm+: positive control (DNA species-specific) and Ctr -: negative control (without DNA), MM – 1 kb Plus Ladder.