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Figure 1 | Malaria Journal

Figure 1

From: The Plasmodium vivax rhoptry neck protein 5 is expressed in the apical pole of Plasmodium vivax VCG-1 strain schizonts and binds to human reticulocytes

Figure 1

In-silico, genomic and transcriptional analysis of the pvron5 gene in the Plasmodium vivax VCG-1 strain. (A) Schematic representation of the pvron5 gene (blue) localized in Pv_Sal1_chr05:629000..649500 (21 kb) and comparative analysis with P. falciparum: Pf3D7_08_v3:798000..818000 (20 kb) and P. knowlesi: Pk_strainH_chr05:663000..682000 (19 kb) and Pk_strainH_chr05:709,700..711,400 (1.7 kB). The transcription direction is shown for each gene and the identity and similarity values between ron5 and adjacent genes regarding the three Plasmodium species. Analysis was made concerning the information available in PlasmoDB [28] (B) pvron5 amplification from gDNA. Above, a representation of the pvron5 gene and the localization of the three sets of primers used for amplifying the gene are shown, along with the size of the expected products. ~3,404 bp (lane 1), ~1,815 bp (lane 2) and ~3,158 bp (lane 3) bands can be observed on the agarose gel, showing the weight of the expected products for pvron5. MWM: molecular weight marker. (C) pvron5 transcription in VCG-1 strain late schizonts. Lane 1: pvron5 positive RT-PCR. Lane 2: pvrhoph3 positive RT-PCR. Lane 3: pvron5 negative RT-PCR.

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Malaria Journal

ISSN: 1475-2875

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