Table 1 Primers used in amplifying and sequencing Pv RON5 from gDNA and cDNA
Name | Sequence 5 → 3 | Amplicon | Target |
|---|---|---|---|
A1 | CGT CTG TAA GAC CTC CC | 3.404 bp | gDNA |
A3 | CGA TGA AGC CCT TCT CC | gDNA | |
A5 | CGG GAC AAG CTG AAT AAC | 1.815 bp | gDNA |
A7 | TGA CGT CGG CGC AGA TG | gDNA | |
A9 | AGT GCC TCC ATG GAC AAT A | 3.158 bp | gDNA |
A11 | GCT GAT CGG TCG GCT GA | gDNA | |
PvRON5-F | ATG CTG AAG TAC GTG CTA CTC | 3.477 bp | cDNA |
PvRON5-R | GGG TAT CCT CGT GTG CAC | cDNA | |
PvRON5-sec-F1 | CGT CTG TAA GAC CTC CC | NA | cDNA sequencing |
PvRON5-rev-R1 | CGA TGA AGC CCT TCT CC | NA | cDNA sequencing |
PvRON5-sec-F2 | CGG GAC AAG CTG AAT AAC | NA | cDNA sequencing |
PvRON5-sec-R2 | TGA CGT CGG CGC AGA TG | NA | cDNA sequencing |
PvRON5-sec-F3 | AGT GCC TCC ATG GAC AAT A | NA | cDNA sequencing |
PvRON5-sec-R3 | GCT GAT CGG TCG GCT GA | NA | cDNA sequencing |