Figure 2

Automated counting can detect binding comparable to manual microscopic examination. A) Increasing densities of parasites were added to wells and the numbers of adherent cells were quantified using both microscopic examination and automated detection. B) The mean value for each tested density for both methods was plotted against each other. The quantifications were highly correlated (r = 0.98, p <0.001). For the regression line, erythrocyte counts were calculated from OD-values in the automated method using a standard curve. C) Bland-Altman plot from a single experiment. Each circle represents the difference in mean quantity of adherent cells when counts from manual, microscopic counting are subtracted from automated reading at different seeding densities. 0 on the y-axis denotes identical result using both methods.