Efficiency and kinetics of
cytokine cDNA amplification. (A) Efficiency of cytokine (IFNγ, IL2, IL6, IL10, IL12, TNFα) cDNA amplification by real-time PCR using pair of primers specifically designed for Saimiri sciureus sequences. The Figure shows the average Ct values after cytokine amplification using serial five-fold dilutions of cDNA from Saimiri peripheral blood mononuclear leukocytes stimulated with ionomycin-PMA. (B) Kinetics of cytokine (IFNγ, IL2, IL6, IL10, IL12, TNFα) cDNA amplification by real-time PCR following in vitro mitogen stimulation. Peripheral blood mononuclear leukocytes from non-infected Saimiri were stimulated in vitro with ionomycin-PMA and mRNA amplification was determined at various timepoints. Results are shown as fold increases in cytokine expression in relation to unstimulated leukocytes.