Prevalence of Plasmodium falciparum anti-malarial resistance-associated polymorphisms in pfcrt, pfmdr1 and pfnhe1 in Muheza, Tanzania, prior to introduction of artemisinin combination therapy

Table 1 Primers and probes sequences used to amplify and detect SNPs in pfmdr1

Position	Primer/Probe	Sequence 5′→ 3′
86	Pfmdr1-86F	CCT TTT TTT ATA TCT GTG TTT GGT G
	Pfmdr1-86R	TTA TTA TCA TGA AAT TGT CCA TCT TG
	Pfmdr1-86N	ROX-AAA GAA CAT GAA TTT AGG TGA TGA-BHQ-2a
	Pfmdr1-86Y	Cy5- AAA GAA CAT GTA TTT AGG TGA TGA-BHQ-2a
184	Pfmdr1-184F	TTA TAA CAA TTT TTA CAT ATG CCA GTT CC
	Pfmdr1-184R	TCT TAT TAC ATA TGA CAC CAC AAA C
	Pfmdr1-184Y	JOE-TTA GGT TTA TAT ATT TGG TCA TTAAT-BHQ-1a
	Pfmdr1-184F	FAM -TTA GGT TTA TTT ATT TGG TCA TTAAT-BHQ-1a
1042	Pfmdr1-1042F	GAA GAA TTA TTG TAA ATG CAG C
	Pfmdr1-1042R	CCT TTT AAG GAC ATT AAT TTT C
	Pfmdr1-1042N	ROX-ATT ATT TAT TAA TAG TTT TGC-BHQ2a
	Pfmdr1-1042D	Cy5-ATT ATT TAT TGA TAG TTT TGC-BHQ2a
1246	Pfmdr1-1246F	TGC AGA AGA TTAT ACT GTA TTT AAT A
	Pfmdr1-1246R	GCA AAC TTA CTA ACA CGT TTA ACA
	Pfmdr1-1246D	JOE- TAT AAC TTA AGA GAT CTT AGA AAC-BHQ-1a
	Pfmdr1-1246Y	FAM-TAT AAC TTA AGA TAT CTT AGA AAC- BHQ-1a

Mutant bases of the Pfmdr1-86Y, Pfmdr1-184F, Pfmdr1-1042D and Pfmdr1-1246Y primers are shown in bold font. ROX, Cy5, JOE, FAM indicate fluorescent reporter dyes; BHQ-1a and BHQ-2a denote Black Hole Quencher Dyes 1a and 2a, respectively.

ISSN: 1475-2875