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Figure 1 | Malaria Journal

Figure 1

From: Assessing anti-malarial drug effects ex vivo using the haemozoin detection assay

Figure 1

Flow cytometry modifications to detect light depolarization. The optical set-up of the Cyflow (Partec, Münster, Germany) (A) was easily modified to allow the detection of light depolarization. Four optical components from the original set-up (blue boxes in A) had to be replaced by the optical components shown in B, as described in the Methods section. Filters were simply replaced by unscrewing the original ones from the filter holder, or by removing the metallic ring (second row in B). This was accomplished using simple tools (bottom row in B). Note that in this case, polarization filters were glued inside the holder (arrows in B) so that they would not move, since to attain the best depolarization signal polarizers should be perpendicular to each other. The final optical layout to detect light depolarization is presented in C.

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