Assessing anti-malarial drug effects ex vivo using the haemozoin detection assay

Malaria Journal

Table 1 Summarized data of isolates exhibiting different ex vivo growth profiles analysed by the haemozoin assay

	Group 1	Group 2	Group 3	Group 4
Growth profile	No maturation	Maturation at 24 hrs and replication at 48 hrs	Maturation at 24 hrs but no replication	Delayed maturation
Number of samples	7	8	17	14
Parasitaemia	0.01%;	0.3%;	3.5%;	0.3%;
(median; range)	0.001-0.1%	0.2-2.2%	0.2-12%	0.1-1.9%
IC50 Artesunate (mean)	nd	10.5 nM	15.2 nM	5.6 nM
IC50 Artemisinin (mean)	nd	46 nM	47.3 nM	14.4 nM

nd – not determined.
IC50 – 50% inhibitory concentration.
Note: Results from chloroquine were not presented in this Table because the majority of the samples (32 out of 46) had IC50 values higher than 200 nM. Chloroquine had an inhibitory effect in only four samples from group 3 (IC50 mean = 76.8 nM). In ten samples, chloroquine activity could not be determined.

ISSN: 1475-2875