Robust, reproducible, industrialized, standard membrane feeding assay for assessing the transmission blocking activity of vaccines and drugs against Plasmodium falciparum

Table 1 Summary of SMFA control results

Polyclonal anti-sera at 1:54 final dilution (number of assays)		Infection intensity (number of oocysts per mosquito)			Infection prevalence (percent of mosquitoes with oocysts)
		Negative control	Pfs25 antibody	TBA-I	Negative control	Pfs25 antibody	TBA-P
MRA39 (n = 44)	Mean ± SD	47.8 ± 32.9	8.4 ± 8.9	81.6 ± 12.9	93.8 ± 7.2	81.9 ± 16.5	12.9 ± 16.6
	95% CI	38.1, 57.5	5.8, 11.1	77.7, 85.4	91.7, 96.0	77.0, 86.8	8.0, 17.8
	Range	3.7, 135.6	0.1, 47.0	36.5, 98.9	71.4, 100.0	8.3, 100.0	−8.7, 89.6
MRA38 (n = 30)	Mean ± SD	43.7 ± 25.8	1.3 ± 1.6	97.0 ± 3.9	94.6 ± 4.4	34.6 ± 27.1	63.5 ± 28.6
	95% CI	34.5, 52.9	0.7, 1.8	95.6, 98.4	92.9, 96.3	24.9, 44.3	53.3, 73.7
	Range	7.1, 106.4	0.0, 4.8	87.4, 100.0	84.2, 100.0	0, 79.2	10.0, 100.0
Pooled data (n = 74)	Mean ± SD	46.1 ± 30.1			94.2 ± 6.3
	95% CI	39.3, 52.5			92.7, 95.6
	Range	3.7, 135.6			71.4, 100.0

The positive and negative control data from 74 independent SMFAs are summarized to show the infection intensity and infection prevalence in mosquitoes fed negative control (human O⁺) serum or positive control anti-Pfs25 antibodies. Transmission blocking activities measured as effects on intensity (TBA-I) and prevalence (TBA-P) are calculated. In each SMFA there was one cage of negative control mosquitoes and one cage of positive control mosquitoes. For infection intensities, data are the arithmetic means ± standard deviation (SD) of the geometric mean intensities from each cage of negative or positive control mosquitoes as well as the 95% confidence intervals (CI) and range. For infection prevalence, data are the arithmetic means ± SD of the percent of mosquitoes infected in each cage of negative or positive control mosquitoes as wells as the 95% CI and range.

ISSN: 1475-2875