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Table 2 Variability of the intron-1 of the kdr gene in Anopheles coluzzii and Anopheles gambiae collected in 2011 in Douala and Yaoundé

From: Rapid evolution of pyrethroid resistance prevalence in Anopheles gambiae populations from the cities of Douala and Yaoundé (Cameroon)

Mutations positions Unpolluted Polluted Cultivated
702 703 896 Others Haplotypes An. coluzzii   An. coluzzii % An. coluzzii An. gambiae
      2 N = 30 % 2 N = 18 % 2 N = 22 % 2 N = 16 %
T C C - MS1 16 53.3% 8 44.4% 4 18.2% 16 100%
T C A - M14 - - 2 11.1% 2 9.09% - -
C C C - MS3 6 20% 6 33.3% 12 54.5% - -
C C C 169C M15 4 13.3% - - - - - -
C C C 394G M16 4 13.3% 2 11.1% 4 18.2% - -
Kdr alleles- Intron-1 haplotypes         
    MS1-L1014L 6 40% 3 33.3% - - 1 12.5%
    MS1-L1014F 2 13.3% 1 11.1% 2 18.2% 6 75%
    MS1-L1014S - - - - - - 1 12.5%
    MS3-L1014L 3 20% 3 33.3% 6 54.5% - -
    M14-L1014L - - 1 11.1% 1 9.1% - -
    M15-L1014L 1 6.67% - - - - - -
    M15-L1014F 1 6.67% - - - - - -
    M16-L1014L 2 13.3% 1 11.1% 2 18.2% - -
   Estimates of genetic variability         
     h 4   4   4   3  
     Hd ± SD 0.82 ± 0.034   0.76 ± 0.05   0.59 ± 0.085   0.43 ± 0.14  
     k 1.42   1.098   1.048   0.633  
     n 4   3   3   2  
     D 1.045   0.718   0.706   0.13  
     Fs −0.614   0.089   1.43   0.062  
  1. h: number of different haplotypes; Hd ± SD, haplotype diversity (±standard deviation); K: average number of pairwise nucleotide differences; n: number of mutations; D: Tajima’s test; Fs: Fu’s Fs statistic.