Skip to main content

Table 2 Anopheles species, number of mosquitoes tested positive for Plasmodium DNA/number of individuals tested for infection (+/n), frequency (%) of infected Anopheles , PCR method (real-time and conventional PCR) and the parasite species per landscape, Atlantic forest, Brazil, August-November 2012

From: Plasmodium falciparum in the southeastern Atlantic forest: a challenge to the bromeliad-malaria paradigm?

Landscape Species a Real-time PCR +/n (%) PCR +/n (%) Parasite species b
1-A An. cruzii 0/6 (0.0) - -
An. fluminensis 0/3 (0.0) - -
An. strodei 1/72 (1.4) 0/1 (0.0) P. falciparum
An. triannulatus 1/12 (8.3) 0/1 (0.0) P. falciparum
Subtotal 2/93 (2.15) 0/2 (0.0) -
1-B An. cruzii 0/22 (0.0) - -
An. bellator 0/9 (0.0) - -
Subtotal 0/31 (0.0) - -
1-C An. cruzii 0/1 (0.0) - -
Subtotal 0/1 (0.0) - -
2 An. albitarsis 0/14 (0.0) - -
Subtotal 0/14 (0.0) - -
3 An. albitarsis 0/1 (0.0) - -
An. cruzii 0/20 (0.0) - -
An. galvaoi 1/48 (2.1) 1/1 (100.0) P. falciparum
An. mediopunctatus 0/42 (0.0) - -
Subtotal 1/111 (0.9) 1/1 (100.0) -
4 An. albitarsis 0/13 (0.0) - -
An. cruzii 0/4 (0.0) - -
An. strodei 0/1 (0.0) - -
An. triannulatus 0/5 (0.0) - -
Subtotal 0/23 (0.0) - -
5 An. cruzii 4/207 (1.9) 1/4 (25.0) P. vivax
An. cruzii 18/207 (8.7) 15/18 (83.0) P. falciparum
Subtotal 22/207 (10.6) 16/22 (72.7) -
  Total P. vivax 4/480 (0.8) 1/4 (25.0)  
  Total P. falciparum 21/480 (4.4) 16/21 (76.2)  
  Total 25/480 (5.2) 17/25 (68.0)  
  1. aMorphological identification of the specimens tested positive for Plasmodium was confirmed by DNA COI barcode sequences.
  2. b Plasmodium species identified in Anopheles mosquitoes by real-time PCR and/or conventional PCR plus DNA sequencing with species-specific primers [21].