|
Landscape
|
Species
a
|
Real-time PCR +/n (%)
|
PCR +/n (%)
|
Parasite species
b
|
|---|
|
1-A
|
An. cruzii
|
0/6 (0.0)
|
-
|
-
|
|
An. fluminensis
|
0/3 (0.0)
|
-
|
-
|
|
An. strodei
|
1/72 (1.4)
|
0/1 (0.0)
|
P. falciparum
|
|
An. triannulatus
|
1/12 (8.3)
|
0/1 (0.0)
|
P. falciparum
|
|
Subtotal
|
2/93 (2.15)
|
0/2 (0.0)
|
-
|
|
1-B
|
An. cruzii
|
0/22 (0.0)
|
-
|
-
|
|
An. bellator
|
0/9 (0.0)
|
-
|
-
|
|
Subtotal
|
0/31 (0.0)
|
-
|
-
|
|
1-C
|
An. cruzii
|
0/1 (0.0)
|
-
|
-
|
|
Subtotal
|
0/1 (0.0)
|
-
|
-
|
|
2
|
An. albitarsis
|
0/14 (0.0)
|
-
|
-
|
|
Subtotal
|
0/14 (0.0)
|
-
|
-
|
|
3
|
An. albitarsis
|
0/1 (0.0)
|
-
|
-
|
|
An. cruzii
|
0/20 (0.0)
|
-
|
-
|
|
An. galvaoi
|
1/48 (2.1)
|
1/1 (100.0)
|
P. falciparum
|
|
An. mediopunctatus
|
0/42 (0.0)
|
-
|
-
|
|
Subtotal
|
1/111 (0.9)
|
1/1 (100.0)
|
-
|
|
4
|
An. albitarsis
|
0/13 (0.0)
|
-
|
-
|
|
An. cruzii
|
0/4 (0.0)
|
-
|
-
|
|
An. strodei
|
0/1 (0.0)
|
-
|
-
|
|
An. triannulatus
|
0/5 (0.0)
|
-
|
-
|
|
Subtotal
|
0/23 (0.0)
|
-
|
-
|
|
5
|
An. cruzii
|
4/207 (1.9)
|
1/4 (25.0)
|
P. vivax
|
|
An. cruzii
|
18/207 (8.7)
|
15/18 (83.0)
|
P. falciparum
|
|
Subtotal
|
22/207 (10.6)
|
16/22 (72.7)
|
-
|
| |
Total P. vivax
|
4/480 (0.8)
|
1/4 (25.0)
| |
| |
Total P. falciparum
|
21/480 (4.4)
|
16/21 (76.2)
| |
| |
Total
|
25/480 (5.2)
|
17/25 (68.0)
| |
-
aMorphological identification of the specimens tested positive for Plasmodium was confirmed by DNA COI barcode sequences.
-
b
Plasmodium species identified in Anopheles mosquitoes by real-time PCR and/or conventional PCR plus DNA sequencing with species-specific primers [21].
