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Table 1 Intra-day assay precision and accuracy for LUME and 97–63 in rat plasma (n = 6)

From: Simultaneous quantification of proposed anti-malarial combination comprising of lumefantrine and CDRI 97–78 in rat plasma using the HPLC–ESI-MS/MS method: application to drug interaction study

 

LUME (ng/mL)

97-63 (ng/mL)

 

3.9

10

100

400

3.9

10

100

400

Day 1

        

Mean

3.9

9.6

97.4

387.3

4.1

10.2

104.0

405.2

SD

0.23

0.22

6.16

16.61

0.28

0.46

4.75

14.16

Precision (%)a

5.78

2.24

6.33

4.29

6.76

4.54

4.56

3.50

Accuracy (%)b

100.17

96.20

97.35

96.83

104.32

109.12

104.04

101.29

Day 2

        

Mean

3.7

9.9

97.9

397.5

3.8

9.9

102.8

415.3

SD

0.27

0.55

6.93

11.74

0.23

0.53

3.99

9.35

Precision (%)a

7.14

5.51

7.07

2.95

6.01

5.27

3.88

2.25

Accuracy (%)b

95.90

99.47

97.92

99.38

99.40

106.52

102.78

103.83

Day 3

        

Mean

3.8

9.5

103.0

415.0

3.8

10.3

104.3

386.5

SD

0.18

0.48

4.00

18.32

0.18

0.49

4.18

10.89

Precision (%)a

4.81

5.06

3.88

4.41

4.81

4.81

4.01

2.82

Accuracy (%)b

98.25

94.93

103.00

103.75

103.93

109.51

104.33

96.63

  1. a Expressed as % RSD = (SD/mean) × 100.
  2. b Calculated as (mean determined concentration/nominal concentration) × 100.