Subcellular localization of PvRALP1 protein in asexual blood-stage parasites of P. vivax. Acetone-fixed mature schizonts of P. vivax were dual labeled with mouse immune sera against PvRALP1-Tr (green), PvRON2 (rhoptry neck marker) (A, red), rabbit immune sera against PvDBP (microneme marker) (B, red), and PvRhopH2 (rhoptry marker) (C, red). Nuclei are visualized with DAPI (blue). DIC; differential interference contrast image. Scale bar represents 5 μm.