Advantages | Disadvantages | |
---|---|---|
AlphaLISA | • High throughput: | • Prone to interferences: aggregating compounds (false positive), chemical or colour quenchers (false negative) |
- Homogeneous: mix and measure assay eliminating washing steps | ||
- Save time and work | ||
- 1,536 well plate format | ||
DELFIA | • Lower interference: | • Lower throughput: |
- Compounds and non-specific assay reagents are removed in the washing steps | - Heterogeneous. Washes are needed: labour intensive | |
- Transfer step: iRBCs transfer to streptavidin plates is required after treatment. Time consuming. | ||
- More sources of assay variability | ||
- 384-well plates |