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Table 1 Advantages and disadvantages of these new AlphaLISA and DELFIA assays

From: Development of two novel high-throughput assays to quantify ubiquitylated proteins in cell lysates: application to screening of new anti-malarials

 

Advantages

Disadvantages

AlphaLISA

• High throughput:

• Prone to interferences: aggregating compounds (false positive), chemical or colour quenchers (false negative)

- Homogeneous: mix and measure assay eliminating washing steps

- Save time and work

- 1,536 well plate format

DELFIA

• Lower interference:

• Lower throughput:

- Compounds and non-specific assay reagents are removed in the washing steps

- Heterogeneous. Washes are needed: labour intensive

- Transfer step: iRBCs transfer to streptavidin plates is required after treatment. Time consuming.

- More sources of assay variability

- 384-well plates