Table 1 Induction of in vitro gametocytogenesis in P. falciparum parasites under various conditions
Conditions | Gametocytaemiaa | Conversion Factorb | |
|---|---|---|---|
Shaking vs. stationary (serum medium) | |||
Asexual culture | Gametocyte culture | Â | |
Stationary | Stationary | 1.3 ± 0.6 % (n = 4) | 15.8 ± 0.6 % |
Shaking | Stationary | 3.3 ± 1.4 % (n = 22) | 34.2 ± 3.4 % |
Medium composition (asexual, stationary gametocyte cultures) | |||
Asexual culture | Gametocyte culture | Â | |
AlbuMAX | AlbuMAX | 5.1 ± 1.0 % (n = 10) | 33.3 ± 1.9 % |
|  | Serum | 1.9 ± 0.8 % (n = 3) | 8.2 ± 2.2 % |
Serum | AlbuMAX | 3.7 ± 1 (n = 3) | 22.7 ± 1.1 % |
|  | Serum | 1.3 ± 0.6 % (n = 4) | 20.7 ± 3.3 % |
Asexual parasite elimination | |||
Using sorbitol | 5.3 ± 0.2 % (n = 2) | 32.3 ± 1.4 % | |
Using NAG | 4.9 ± 1.0 % (n = 2) | 37.7 ± 1.7 % | |
Different strains of P. falciparum parasites | |||
NF54 | 4.9 ± 1.0 % (n = 3) | 35.0 ± 1.7 % | |
3D7 | 1.5 ± 0.2 % (n = 3) | 11.2 ± 2.0 % | |
W2 | 1.1 ± 0.2 % (n = 3) | 8.9 ± 0.5 % | |
7G8 | 1 ± 0.7 % (n = 2) | ND | |
FCR3 | 0 % (n = 3) | NA | |
HB3 | 0 % (n = 3) | NA | |