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Table 1 Induction of in vitro gametocytogenesis in P. falciparum parasites under various conditions

From: Nowhere to hide: interrogating different metabolic parameters of Plasmodium falciparum gametocytes in a transmission blocking drug discovery pipeline towards malaria elimination

Conditions Gametocytaemiaa Conversion Factorb
Shaking vs. stationary (serum medium)
Asexual culture Gametocyte culture  
Stationary Stationary 1.3 ± 0.6 % (n = 4) 15.8 ± 0.6 %
Shaking Stationary 3.3 ± 1.4 % (n = 22) 34.2 ± 3.4 %
Medium composition (asexual, stationary gametocyte cultures)
Asexual culture Gametocyte culture  
AlbuMAX AlbuMAX 5.1 ± 1.0 % (n = 10) 33.3 ± 1.9 %
  Serum 1.9 ± 0.8 % (n = 3) 8.2 ± 2.2 %
Serum AlbuMAX 3.7 ± 1 (n = 3) 22.7 ± 1.1 %
  Serum 1.3 ± 0.6 % (n = 4) 20.7 ± 3.3 %
Asexual parasite elimination
Using sorbitol 5.3 ± 0.2 % (n = 2) 32.3 ± 1.4 %
Using NAG 4.9 ± 1.0 % (n = 2) 37.7 ± 1.7 %
Different strains of P. falciparum parasites
NF54 4.9 ± 1.0 % (n = 3) 35.0 ± 1.7 %
3D7 1.5 ± 0.2 % (n = 3) 11.2 ± 2.0 %
W2 1.1 ± 0.2 % (n = 3) 8.9 ± 0.5 %
7G8 1 ± 0.7 % (n = 2) ND
FCR3 0 % (n = 3) NA
HB3 0 % (n = 3) NA
  1. ND not detected / determined, NA not applicable
  2. a Day 11 after induction
  3. b \( \mathrm{Conversion}\ \mathrm{factor}=\kern0.5em \frac{\mathrm{Number}\ \mathrm{of}\ \mathrm{stage}\ 2\ \mathrm{gametocytes}\ \mathrm{on}\ \mathrm{day}\ 4}{\mathrm{Number}\ \mathrm{of}\ \mathrm{rings}\ \mathrm{on}\ \mathrm{day}\ 2} \)
  4. Data are representative of (n) biological experiments, each performed in triplicate, ± SEM. Data indicate maximal levels of gametocytaemia obtained