Fig. 9
Haem fractionation profiles for amodiaquine-treated parasites. The percentages of ‘free’ haem (a) and Hz (b) are shown at increasing concentrations of AQ. The parasite survival curve for AQ, determined using the parasite lactate dehydrogenase assay [22] overlaid with the percentage of ‘free’ haem shows a rise in ‘free’ haem with increasing AQ concentration corresponding to decreased parasite survival (c). The total haem Fe per trophozoite of all AQ-treated cells is not statistically different to untreated cells (d). The amount of ‘free’ haem Fe (e) and Hz Fe (f) are shown at increasing concentrations of AQ. The parasite survival curve for AQ, overlaid with the amount of ‘free’ haem shows a rise in ‘free’ haem with increasing AQ concentration correlated to decreased parasite survival (G). AQ IC50 in D10 = 21.6 ± 3.0 nM