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Table 1 Number of natural copulations recorded for different thermoperiod/light induction periods for An. darlingi enclosed in cages of two different sizes (medium = 46 × 46 ×46 cm; large = 61 × 61 × 61 cm) and with different adult densities

From: Establishment of a free-mating, long-standing and highly productive laboratory colony of Anopheles darlingi from the Peruvian Amazon

Period of natural copulation induction (days)

Medium cage

Large cage

1,500 adults

2,400 adults

(1:1 ♂:♀)

(1:1 ♂:♀)

1

5

10

2

8

65

3

10

60

4

15

100

5

8

4

6

4

11

7

5

5

Total number of copulations

55

255

  1. F1 adults tested were 2–3 days old. Observations were conducted in the NAMRU-6 insectary in Iquitos, Peru