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Table 1 Number of natural copulations recorded for different thermoperiod/light induction periods for An. darlingi enclosed in cages of two different sizes (medium = 46 × 46 ×46 cm; large = 61 × 61 × 61 cm) and with different adult densities

From: Establishment of a free-mating, long-standing and highly productive laboratory colony of Anopheles darlingi from the Peruvian Amazon

Period of natural copulation induction (days) Medium cage Large cage
1,500 adults 2,400 adults
(1:1 ♂:♀) (1:1 ♂:♀)
1 5 10
2 8 65
3 10 60
4 15 100
5 8 4
6 4 11
7 5 5
Total number of copulations 55 255
  1. F1 adults tested were 2–3 days old. Observations were conducted in the NAMRU-6 insectary in Iquitos, Peru