Table 2 Total numbers of eggs, larvae, pupae, and adults; and percentage of unhatched eggs, larval, and pupal mortality of An. darlingi reared for 19 generations (F1–F19) in the NAMRU-6 insectary in Iquitos, Peru
Generation | No. of cages | Copulation induction period (days) | Total eggs | Unhatched eggs (%) | Total larvae | Larva-pupa mortality (%) | Total pupae | Pupa-adult mortality (%) | Total adultsa |
|---|---|---|---|---|---|---|---|---|---|
1 | 4 | 5 | 5,000 | 4.0 | 4,800 | 4.2 | 4,600 | 8.0 | 4,230 |
2 | 4 | 5 | 10,000 | 19.4 | 8,063 | 0.9 | 7,993 | 5.0 | 7,593 |
3 | 3 | 5 | 10,590 | 61.3 | 4,095 | 5.8 | 3,859 | 4.0 | 3,704 |
4 | 8 | 5 | 29,380 | 48.9 | 15,000 | 44.5b | 8,327 | 1.3 | 8,216 |
5 | 3 | 5 | 35,419 | 22.0 | 27,616 | 93.3c | 1,845 | 34.0c | 1,218 |
6 | 3 | 5 | 14,560 | 55.0 | 6,550 | 51.4 | 3,184 | 6.2 | 2,987 |
7 | 2 | 5 | 18,790 | 33.5 | 12,500 | 47.6 | 6,544 | 8.3 | 6,000 |
8 | 2 | 5 | 4,950 | 29.3 | 3,500 | 54.7 | 1,586 | 6.4 | 1,485 |
9 | 7 | 0/5d | 24,000 | 47.9 | 12,500 | 35.4 | 8,073 | 7.6 | 7,460 |
10 | 10 | 0/3e | 43,810 | 44.1 | 24,500 | 11.8 | 21,619 | 6.5 | 20,205 |
11 | 7 | 0 | 46,600 | 57.1 | 20,000 | 13.3 | 17,339 | 4.9 | 16,485 |
12 | 6 | 0 | 45,450 | 45.0 | 25,000 | 4.8 | 23,790 | 8.9 | 21,664 |
13 | 5 | 0 | 49,130 | 49.1 | 25,000 | 40.1 | 14,976 | 9.0 | 13,629 |
14 | 8 | 0 | 61,750 | 51.4 | 30,000 | 86.1c | 4,183 | 21.4c | 3,289 |
15 | 6 | 0 | 54,000 | 55.6 | 24,000 | 46.0 | 12,951 | 8.9 | 11,801 |
16 | 9 | 0 | 116,465 | 59.6 | 47,000 | 54.7 | 21,272 | 7.0 | 19,773 |
17 | 6 | 0 | 57,100 | 29.9 | 40,000 | 82.2c | 7,130 | 11.0 | 6,345 |
18 | 6 | 0 | 53,880 | 33.2 | 36,000 | 67.2 | 11,820 | 6.5 | 11,057 |
19 | 7 | 0 | 82,901 | 9.5 | 75,000 | 10.7 | 66,950 | 3.7 | 64,450 |
TOTAL | 763,775 | 441,124 | 248,041 | 231,591 | |||||
MEAN (SD) | 40,199 (28,594) | 39.8 (17.1) | 23,217 (17,756) | 39.7 (29.6) | 13,055 (14,765) | 8.9 (7.3) | 12,189 (14,202) | ||
95 % CI | 26,417–53,981 | 31.5–48.0 | 14,659–31,775 | 25.5–54.0 | 5,938–20,171 | 5.4–12.4 | 5,344–19,034 |