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Table 2 Total numbers of eggs, larvae, pupae, and adults; and percentage of unhatched eggs, larval, and pupal mortality of An. darlingi reared for 19 generations (F1–F19) in the NAMRU-6 insectary in Iquitos, Peru

From: Establishment of a free-mating, long-standing and highly productive laboratory colony of Anopheles darlingi from the Peruvian Amazon

Generation No. of cages Copulation induction period (days) Total eggs Unhatched eggs (%) Total larvae Larva-pupa mortality (%) Total pupae Pupa-adult mortality (%) Total adultsa
1 4 5 5,000 4.0 4,800 4.2 4,600 8.0 4,230
2 4 5 10,000 19.4 8,063 0.9 7,993 5.0 7,593
3 3 5 10,590 61.3 4,095 5.8 3,859 4.0 3,704
4 8 5 29,380 48.9 15,000 44.5b 8,327 1.3 8,216
5 3 5 35,419 22.0 27,616 93.3c 1,845 34.0c 1,218
6 3 5 14,560 55.0 6,550 51.4 3,184 6.2 2,987
7 2 5 18,790 33.5 12,500 47.6 6,544 8.3 6,000
8 2 5 4,950 29.3 3,500 54.7 1,586 6.4 1,485
9 7 0/5d 24,000 47.9 12,500 35.4 8,073 7.6 7,460
10 10 0/3e 43,810 44.1 24,500 11.8 21,619 6.5 20,205
11 7 0 46,600 57.1 20,000 13.3 17,339 4.9 16,485
12 6 0 45,450 45.0 25,000 4.8 23,790 8.9 21,664
13 5 0 49,130 49.1 25,000 40.1 14,976 9.0 13,629
14 8 0 61,750 51.4 30,000 86.1c 4,183 21.4c 3,289
15 6 0 54,000 55.6 24,000 46.0 12,951 8.9 11,801
16 9 0 116,465 59.6 47,000 54.7 21,272 7.0 19,773
17 6 0 57,100 29.9 40,000 82.2c 7,130 11.0 6,345
18 6 0 53,880 33.2 36,000 67.2 11,820 6.5 11,057
19 7 0 82,901 9.5 75,000 10.7 66,950 3.7 64,450
TOTAL    763,775   441,124   248,041   231,591
MEAN (SD)    40,199 (28,594) 39.8 (17.1) 23,217 (17,756) 39.7 (29.6) 13,055 (14,765) 8.9 (7.3) 12,189 (14,202)
95 % CI    26,417–53,981 31.5–48.0 14,659–31,775 25.5–54.0 5,938–20,171 5.4–12.4 5,344–19,034
  1. aMean sex ratio (male:female) across generations is 1:1
  2. bPresence of Enterobacter sp. and Serratia marcescens
  3. cPresence of fungus and protists
  4. dCopulation induction performed for 5 days only in 4 cages
  5. eCopulation induction performed for 3 days only in 2 cages