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Fig. 1 | Malaria Journal

Fig. 1

From: Development of a genetic tool for functional screening of anti-malarial bioactive extracts in metagenomic libraries

Fig. 1

DS4 coding gene and F076_DS4 construct development. Scaled diagram of the gene designed to control DS4 anti-malarial peptide expression from the arabinose inducible promoter (PBAD). The final construction in fosmid F076_DS4 harboring the ds4 gene in the metagenomic insert is also shown. araO2, araO1 and araI1-I2: promoter regulator regions; CAP: catabolite activator protein binding site; RBS: ribosome binding site; rrnB term: RNA B transcriptional termination region; MI: metagenomic insert; pCC2FOS: fosmid vector. Horizontal black arrows represent all open reading frames (ORFs) predicted in the MI with a minimum length of 450 pb, that started only with ATG

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