Fig. 2

DS4 coding gene transcription from different episomal DNAs. a DS4 CDS amplification from cDNAs of bacterial cultures grown in the presence or absence of L-arabinose: 1 - E. coli Epi300 pCC2FOS_F076; 2 - E. coli Epi300 pUC57; 3 - E. coli Epi300 F076_DS4; 4 - E. coli Epi300 pUC57_DS4. +, indicate the cultures that were induced with L-arabinose, while the rest of the samples did not include the inducer. b DS4 CDS amplification from cDNAs from E. coli Epi300 F076_DS4, after being incubated with the repressor (D-glucose) or the inductor (L-arabinose) of gene transcription. -, PCR mix without DNA; +, positive control for the PCR (with pUC57_DS4 as a template); 1, cDNA from bacterial culture incubated with D-glucose; 2, cDNA from bacterial culture incubated with L-arabinose; *, RT-PCR reactions without reverse transcriptase; MWM, molecular weight marker