Fig. 1

Systematic extracellular protein interaction screening by AVEXIS identified PfMSP7 as a ligand for human P-selectin. a The entire ectodomains of the 17 named P. falciparum merozoite proteins were screened as highly avid β-lactamase-tagged prey proteins against P-selectin bait using the AVEXIS method. Prey binding was quantified by β-lactamase hydrolysis of a colorimetric substrate at 485 nm. P-selectin bound clearly and specifically to PfMSP7. Positive (+) represents mean value for total capture of all preys with an anti-prey bait antibody. b PfMSP7 and P-selectin prey proteins were screened against PfMSP7 and P-selectin bait proteins as well as an OX68 positive control bait (+) and a rat Cd4 negative control bait (−) by AVEXIS. Interactions were detected in both bait:prey orientations. c An anti-P-selectin mAb blocked the interaction between P-selectin bait and PfMSP7 prey in a dose-dependent manner relative to an anti-Cd4 mAb control, which bound to the tag region of the P-selectin protein, in an AVEXIS assay. Bars in (b) and (c) represent means ± SD, n = 3. Representative experiment of more than three independent experiments are shown