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Fig. 2 | Malaria Journal

Fig. 2

From: P-selectin is a host receptor for Plasmodium MSP7 ligands

Fig. 2

PfMSP7 interacted with P-selectin at the surface of cells. a Schematic representing the experimental design, whereby recombinant, pentameric PfMSP7 proteins interact with eGFP-tagged P-selectin at the surface of transfected cells. b The interaction of P-selectin-eGFP transfected cells with FLAG-tagged pentameric PfMSP7 was detected by flow cytometry. Pre-incubation of cells with anti-Cd200R negative control mAb did not impair interaction detection. c P-selectin-GFP transfected cells incubated with CLB-thromb/6 anti P-selectin mAb prior to incubation with FLAG-tagged pentameric PfMSP7 could not replicate this interaction observed in (b)

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