Fig. 4

The PfMSP7 interaction site is located within the C-type lectin and EGF domains of P-selectin. a A schematic showing the extent of the six P-selectin bait truncations that were expressed and then screened for binding to a full-length PfMSP7 prey using the AVEXIS assay. The shortest regions that retained PfMSP7 binding encompassed both the CTL and EGF domains. b P-selectin bait was screened against PfMSP7 fragment prey proteins by AVEXIS. Schematic shows the regions of the PfMSP7 proteins used in this assay, alongside the scheme of PfMSP7 processing described in the text. Bars represent mean + SD n = 3. Representative experiments of two independent experiments are shown. In each experiment the positive controls (+) were PfMSP7 preys screened against OX68 bait and negative controls (−) were PfMSP7 preys screened against the Cd4 tag bait