Figure 4

Membrane topology of RIFINs at the Maurer’s clefts. a Trophozoites of the rosetting strain FCR3S1.2 were analysed by protease protection assay. Intact cells (lanes 1 and 2) or cells permeabilized by hypotonic lysis (HL) or saponin (Sap) were treated with trypsin (+) or left untreated (−). Proteins of 1 × 10⁷ cells were separated by SDS-PAGE and visualized by immunoblotting using α-RIF40.1 and α-RIF29 as well as α-SBP1-NT, α-Spectrin, and α-Glycophorin A/B antisera to control experimental performance. b The α-RIF29 antiserum stains mainly Maurer’s clefts in immunofluorescence assay of 3D7 parasites at different stages (green). Nuclei were stained with Hoechst33342 (blue).