Table 1 Forward and reverse primers, hybridization temperature and MgCL2 concentration used for polymerase chain reaction and sequence analysis
Gene | Forward Primer Reverse Primer | Tm; [MgCl2] |
|---|---|---|
PftetQ | 5′-ATGTTTAAAAGAGTTTACGTATATAAAAATTC 5′-GAAATGTTCATAAGAAATTGGTATATTATT | 50°C; 3 mM |
5′-TTGTATGATAAATCCTAAACCAGATA 5′-TTCATCGTCCTTCTCACAAATTATAT | 50°C; 3 mM | |
5′-TCACGACAAATGTGCTAGATAC 5′-ATCATCATTTGTGGTGGATATATACAT | 55°C; 3 mM | |
5′-TTAAATATTTCAGATAACCTGGATAAAGA 5′-TTGGGATACACTTTATATATTAACACTTTT | 55°C; 3 mM | |
PfssurRNA | 5′-AAAGAATATCAAAGGCGAAAGC 5′-ACCCTTATCAAGAGTATGTTTTAACC | 60°C; 4 mM |
5′-CTACTAGGGTATCTAATCCTATTTGCTA 5′-GATAGTAGTTCAATTCTACTTATTTCCAT | 60°C; 2 mM | |
PflsurRNA | 5′-TATAGACCCGAAATCAAATGATCTAATT 5′-TTTGGACCGTTATAGATACAGCCG | 60°C; 3 mM |
5′-ACATCTGCCCAGTGCTATTATGTTA 5′-AGCTAATGGTGAGATTTGAACTCATAA | 60°C; 3 mM | |
Pfrps7 | 5′-TTTCCATGATCTACATGCCCTA 5′-TTCTTTAGATTCAACTGGGGTTTT | 60°C; 4 mM |