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Figure 6 | Malaria Journal

Figure 6

From: Isolation, production and characterization of fully human monoclonal antibodies directed to Plasmodium falciparum MSP10

Figure 6

Dotblots with native and reduced EGF-like domains of P. falciparum merozoite surface proteins. Native proteins MSP119, DsRed-fusion proteins of MSP8(EGF1), MSP8(EGF2), MSP4(EGF), MSP10(EGF1) and MSP10(EGF2), multi-EGF and DsRed were applied in the indicated amounts. Proteins containing any of the EGF-like domains of MSP10 were also reduced and alkylated and used in the same quantity. The Ghanaian plasma pool (a) and the naïve plasma pool (b) were used in a 1:500 dilution. Recombinantly expressed and purified humAb10.1 (c), humAb10.2 (d) and humAb10.3 (e) were used in concentrations of 100, 50 and 1.4 µg/ml, respectively. As secondary antibody, goat-anti-human IgG-AP (Dianova) at a dilution of 1:5,000 was used.

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