Figure 8

In vitro GIA of P. falciparum 3D7A. Plasmodium falciparum 3D7A had been synchronized three times prior to the start of the GIA at schizont stage. Recombinant humAb10.1 (black triangles), humAb10.2 (green circles) and humAb10.3 (red squares) have been purified by Protein A chromatography, concentrated, re-buffered in culture medium, sterilized by filtration and were applied in final concentrations of up to 10 mg/ml. The plates were harvested after 44 h and the growth of parasites estimated by a pLDH assay. All tests were carried out in technical triplicates; error bars correspond to standard deviation values.